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Image Search Results
Journal: Life Science Alliance
Article Title: Gene-selective transcription promotes the inhibition of tissue reparative macrophages by TNF
doi: 10.26508/lsa.202101315
Figure Lengend Snippet: (A, B, C) RNA from wild-type BMDMs left untreated (Ctrl), stimulated with IL4/IL13, IL4/IL13 + TNF (A), IL4/IL13 + IL1b (B) or IL4/IL13 + IL36 (C) was used for qRT-PCR. Data shown are the mean fold-increase compared with the Ctrl group. (D) TnfR1 −/− BMDMs were stimulated with IL4/IL13 or IL4/IL13 + TNF over time. RNA was isolated and analyzed for Retnla expression. Data shown are the mean fold-increase of the Ctrl group. All values are means ± SEM; **** P < 0.0001. Statistically significant differences were determined by one-way ANOVA with Tukey correction (A, B, C) or by two-way ANOVA (D). If not indicated otherwise superscripts show statistical significance compared with the control group. n = 3 biological replicates.
Article Snippet: Where indicated, BMDMs were stimulated with 10 ng/ml IL4 (produced in insect cells), 10 ng/ml IL13 (210-13; Peprotech), 10 ng/ml TNF (315-01A; Peprotech), 10 ng/ml IL1β (401-ML/CF; R&D System), 33.3 ng/ml IL36α (7059-ML/CF; R&D Systems), 33.3 ng/ml
Techniques: Quantitative RT-PCR, Isolation, Expressing, Control